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fluorescent membrane tension probe er flipper tr  (Spirochrome)


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    Structured Review

    Spirochrome fluorescent membrane tension probe er flipper tr
    (A) Nuclear lamina labeled by lamin A chromobody, skeletonization of the lamina, and discretized lamina points showing regions of positive (green) and negative curvature (red) in infected Vero cells at 8 hpi. (B) The fraction of negative curvature points for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (C) A zoom-in of the nuclear lamina, showing its motion and maximum intensity projection of the skeleton during 24.8 s. Scale bars, 5 µm. (D) Nuclear lamina mobility, measured as the area covered by the skeleton during one minute for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (E) Nuclear envelope (NE) tension analysis by fluorescence lifetime imaging microscopy (FLIM) of noninfected and HSV-1 17 + wild-type virus-infected Vero cells at 12 hpi using fluorescence lifetime-reporting membrane tension probe ER <t>Flipper-TR.</t> A fast FLIM image presented in a pseudocolor scale representing average photon arrival times in each pixel, ranging from 2.0 to 2.6 ns (calibration bar). Representative cells with average lifetime distribution, segmented NE (red) around the Hoechst signal (grey), and average lifetime at the NE region are shown. Scale bar, 10 µm. (F) The longer lifetime component of the ER Flipper probe, responsive to the membrane tension, in noninfected and infected cells at 8 and 12 hpi (n = 51, 58, and 59, respectively). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05) or **** (p < 0.0001). Nonsignificant differences (p ≥ 0.05) are not labeled.
    Fluorescent Membrane Tension Probe Er Flipper Tr, supplied by Spirochrome, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent membrane tension probe er flipper tr/product/Spirochrome
    Average 94 stars, based on 13 article reviews
    fluorescent membrane tension probe er flipper tr - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Nucleus softens during herpesvirus infection"

    Article Title: Nucleus softens during herpesvirus infection

    Journal: PLOS Pathogens

    doi: 10.1371/journal.ppat.1013873

    (A) Nuclear lamina labeled by lamin A chromobody, skeletonization of the lamina, and discretized lamina points showing regions of positive (green) and negative curvature (red) in infected Vero cells at 8 hpi. (B) The fraction of negative curvature points for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (C) A zoom-in of the nuclear lamina, showing its motion and maximum intensity projection of the skeleton during 24.8 s. Scale bars, 5 µm. (D) Nuclear lamina mobility, measured as the area covered by the skeleton during one minute for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (E) Nuclear envelope (NE) tension analysis by fluorescence lifetime imaging microscopy (FLIM) of noninfected and HSV-1 17 + wild-type virus-infected Vero cells at 12 hpi using fluorescence lifetime-reporting membrane tension probe ER Flipper-TR. A fast FLIM image presented in a pseudocolor scale representing average photon arrival times in each pixel, ranging from 2.0 to 2.6 ns (calibration bar). Representative cells with average lifetime distribution, segmented NE (red) around the Hoechst signal (grey), and average lifetime at the NE region are shown. Scale bar, 10 µm. (F) The longer lifetime component of the ER Flipper probe, responsive to the membrane tension, in noninfected and infected cells at 8 and 12 hpi (n = 51, 58, and 59, respectively). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05) or **** (p < 0.0001). Nonsignificant differences (p ≥ 0.05) are not labeled.
    Figure Legend Snippet: (A) Nuclear lamina labeled by lamin A chromobody, skeletonization of the lamina, and discretized lamina points showing regions of positive (green) and negative curvature (red) in infected Vero cells at 8 hpi. (B) The fraction of negative curvature points for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (C) A zoom-in of the nuclear lamina, showing its motion and maximum intensity projection of the skeleton during 24.8 s. Scale bars, 5 µm. (D) Nuclear lamina mobility, measured as the area covered by the skeleton during one minute for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (E) Nuclear envelope (NE) tension analysis by fluorescence lifetime imaging microscopy (FLIM) of noninfected and HSV-1 17 + wild-type virus-infected Vero cells at 12 hpi using fluorescence lifetime-reporting membrane tension probe ER Flipper-TR. A fast FLIM image presented in a pseudocolor scale representing average photon arrival times in each pixel, ranging from 2.0 to 2.6 ns (calibration bar). Representative cells with average lifetime distribution, segmented NE (red) around the Hoechst signal (grey), and average lifetime at the NE region are shown. Scale bar, 10 µm. (F) The longer lifetime component of the ER Flipper probe, responsive to the membrane tension, in noninfected and infected cells at 8 and 12 hpi (n = 51, 58, and 59, respectively). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05) or **** (p < 0.0001). Nonsignificant differences (p ≥ 0.05) are not labeled.

    Techniques Used: Labeling, Infection, Fluorescence, Imaging, Microscopy, Virus, Membrane, Standard Deviation



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    fluorescent membrane tension probe er flipper tr  (Spirochrome)
    94
    Spirochrome fluorescent membrane tension probe er flipper tr
    (A) Nuclear lamina labeled by lamin A chromobody, skeletonization of the lamina, and discretized lamina points showing regions of positive (green) and negative curvature (red) in infected Vero cells at 8 hpi. (B) The fraction of negative curvature points for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (C) A zoom-in of the nuclear lamina, showing its motion and maximum intensity projection of the skeleton during 24.8 s. Scale bars, 5 µm. (D) Nuclear lamina mobility, measured as the area covered by the skeleton during one minute for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (E) Nuclear envelope (NE) tension analysis by fluorescence lifetime imaging microscopy (FLIM) of noninfected and HSV-1 17 + wild-type virus-infected Vero cells at 12 hpi using fluorescence lifetime-reporting membrane tension probe ER <t>Flipper-TR.</t> A fast FLIM image presented in a pseudocolor scale representing average photon arrival times in each pixel, ranging from 2.0 to 2.6 ns (calibration bar). Representative cells with average lifetime distribution, segmented NE (red) around the Hoechst signal (grey), and average lifetime at the NE region are shown. Scale bar, 10 µm. (F) The longer lifetime component of the ER Flipper probe, responsive to the membrane tension, in noninfected and infected cells at 8 and 12 hpi (n = 51, 58, and 59, respectively). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05) or **** (p < 0.0001). Nonsignificant differences (p ≥ 0.05) are not labeled.
    Fluorescent Membrane Tension Probe Er Flipper Tr, supplied by Spirochrome, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent membrane tension probe er flipper tr/product/Spirochrome
    Average 94 stars, based on 1 article reviews
    fluorescent membrane tension probe er flipper tr - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Nuclear lamina labeled by lamin A chromobody, skeletonization of the lamina, and discretized lamina points showing regions of positive (green) and negative curvature (red) in infected Vero cells at 8 hpi. (B) The fraction of negative curvature points for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (C) A zoom-in of the nuclear lamina, showing its motion and maximum intensity projection of the skeleton during 24.8 s. Scale bars, 5 µm. (D) Nuclear lamina mobility, measured as the area covered by the skeleton during one minute for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (E) Nuclear envelope (NE) tension analysis by fluorescence lifetime imaging microscopy (FLIM) of noninfected and HSV-1 17 + wild-type virus-infected Vero cells at 12 hpi using fluorescence lifetime-reporting membrane tension probe ER Flipper-TR. A fast FLIM image presented in a pseudocolor scale representing average photon arrival times in each pixel, ranging from 2.0 to 2.6 ns (calibration bar). Representative cells with average lifetime distribution, segmented NE (red) around the Hoechst signal (grey), and average lifetime at the NE region are shown. Scale bar, 10 µm. (F) The longer lifetime component of the ER Flipper probe, responsive to the membrane tension, in noninfected and infected cells at 8 and 12 hpi (n = 51, 58, and 59, respectively). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05) or **** (p < 0.0001). Nonsignificant differences (p ≥ 0.05) are not labeled.

    Journal: PLOS Pathogens

    Article Title: Nucleus softens during herpesvirus infection

    doi: 10.1371/journal.ppat.1013873

    Figure Lengend Snippet: (A) Nuclear lamina labeled by lamin A chromobody, skeletonization of the lamina, and discretized lamina points showing regions of positive (green) and negative curvature (red) in infected Vero cells at 8 hpi. (B) The fraction of negative curvature points for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (C) A zoom-in of the nuclear lamina, showing its motion and maximum intensity projection of the skeleton during 24.8 s. Scale bars, 5 µm. (D) Nuclear lamina mobility, measured as the area covered by the skeleton during one minute for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (E) Nuclear envelope (NE) tension analysis by fluorescence lifetime imaging microscopy (FLIM) of noninfected and HSV-1 17 + wild-type virus-infected Vero cells at 12 hpi using fluorescence lifetime-reporting membrane tension probe ER Flipper-TR. A fast FLIM image presented in a pseudocolor scale representing average photon arrival times in each pixel, ranging from 2.0 to 2.6 ns (calibration bar). Representative cells with average lifetime distribution, segmented NE (red) around the Hoechst signal (grey), and average lifetime at the NE region are shown. Scale bar, 10 µm. (F) The longer lifetime component of the ER Flipper probe, responsive to the membrane tension, in noninfected and infected cells at 8 and 12 hpi (n = 51, 58, and 59, respectively). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05) or **** (p < 0.0001). Nonsignificant differences (p ≥ 0.05) are not labeled.

    Article Snippet: Nuclear membrane tension was analyzed by FLIM using fluorescent membrane tension probe ER Flipper-TR (Spirochrome) in noninfected and HSV-1 17 + wt infected live Vero cells at 8 and 12 hpi.

    Techniques: Labeling, Infection, Fluorescence, Imaging, Microscopy, Virus, Membrane, Standard Deviation